Topics Approaches    

Approaches

Cell Culture and Manipulation

Cell Imaging

Computational Modeling of Cell Shape Change and Migration

 


Facilities

General

 

* Chemical and Protein Preparation Facilities * Cell Culture Facilities
* DNA Preparation Facilities with PCR Thermocycler * Amaxa Nucleofector
* Microfabrication and Micropatterning Facilities * Fluorescence Activated Cell Sorting (shared facility)

Optics

 
* Multiple Microscope Imaging Workstations * Microscope Stage Incubators
* Electron-Multiplication CCD Cameras * Precision Motorized stages and Auto-Focusing
* Spinning Disk Confocal Head * Total-Internal Reflection Fluorescence Optics
* Photobleaching System * Atomic Force Microscope (shared facility)
* Stochastic Optical Reconstruction Microscopy (shared facility)

Single Cell Micromanipulation

 
* Micromanipulators, signle- and double-instruments * Custom Microinjection Pressure Regulators
* Micropipette Puller * Microforge

Computation and Image Processing

 
* Linux Workstation for Traction Force Microscopy * Lab Cloud Data Storage System
* Access to Cloud Computing System  
* Custom Software for  
- Multi-Target Timelapse Image Data Collection - Auto-Focusing
- Deconvolution - Moving Target Tracking
- Denoising - Optical Flow/PIV Analysis
- Temporal Differential Microscopy - Spatial Temporal Image Correlation Analysis