Cytoplasmic streaming in amoeboid movement is believed to be driven by a gradient of pressure, which in turn may be generated by localized contractions in the tail region. In cultured adherent cells, neither cytoplasmic streaming nor force gradient has been reported, due in part to the difficulties in detecting intracellular forces. This problem was addressed by microinjecting non-crosslinked, high molecular weight, linear polyacrylamide (PAA) polymers into migrating 3T3 fibroblasts. The polymers form tangled aggregates, which move and change shapes in response to the distribution of surrounding forces. |