| Topics | Approaches |
Approaches
Cell Culture and Manipulation
- 2D and 3D cell culture
- Transfection and gene silencing
- Substrate micropatterning
- Complex culture environment generated with 3D printing
- Single cell mechanical probing and stimulation
- Single cell microinjection, micromanipulation, and microsurgery
- Microfluidic drug release
Cell Imaging
- Phase, DIC, fluorescence imaging,confocal fluorescence Imaging
- Long-term multi-position live cell time-lapse imaging with auto-focusing and cell tracking
- Total internal reflection fluorescence imaging
- Photobleaching and photoablation
- Optical sectioning and tile imaging
- Digital image processing and analysis Including deconvolution, feature extraction, and denoising
Computation
- Traction Force Microscopy, conventional and machine learning based
- 3D image reconstruction
- Modeling of cell migration
Facilities
General |
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| * Tabletop Ultracentrifuge (left) | * Cell Culture Facilities and cell counter | ||
| * PCR Thermocycler (center left) | * Amaxa Nucleofector (center right) | ||
| * Microfabrication and Micropatterning Facilities | * Plasma Generator | ||
| * SLA 3D Printers (right) | * Plate Reader (floor facility) | ||
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Optics |
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| * Multiple Microscope Imaging Workstations (left) | * Microscope Stage Incubators | ||
| * Electron-Multiplication CCD Cameras (center right) | * Precision Motorized stages and Auto-Focusing | ||
| * Spinning Disk Confocal Head (center left) | * Total-Internal Reflection Fluorescence Optics | ||
| * Photobleaching System | * Atomic Force Microscope (floor facility, right) | ||
| *Scanning laser confocal microscopes (floor facility) | |||
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Single Cell Micromanipulation |
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| * Micromanipulators (left) | * Custom Microinjection Pressure Regulators (center right) | ||
| * Micropipette Puller (center left) | * Microforge (right) | ||
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Computation and Image Processing |
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| - Multi-Position Timelapse Image Data Collection | - Auto-Focusing | ||
| - Deconvolution | - AI-Driven Cell Tracking | ||
| - Denoising | - Optical Flow/PIV Analysis | ||
| - Temporal Differential Microscopy | - Spatial Temporal Image Correlation Analysis | ||