Unlike myosin II, actin shows a striking flux toward the equator during early cytokinesis. The juxtaposition of stationary and moving structures argues against a global cortical flow process. The left panel was recorded with a cell expression GFP-actin. The right panel, recorded with cells expressing m-Cherry actin, shows highly active ruffling in a neighboring cell in addition very active flux. Unlike myosin, actin shows no dynamic domains of assembly. Recording time, 134 sec (left), and 70 sec (right). |